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Variability in microRNA recovery from plasma: However, the relative expression levels for miR, miRa, and let-7a kits differed depending on the kit used. Comparison of five commercial kits, doi: Appendix A Supplementary data associated with this article can be found in the online version at doi: Experimental factors CRC patients vs. Author information Article notes Copyright and License information Disclaimer.

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Articles from Data in Brief are provided here courtesy of Elsevier. Data accessibility Data found in this article. Constant amplification efficiency in all compared samples is indispensable when relative quantification is used to measure changes in gene expression. However, the relative expression levels for miR, miRa, and let-7a kits differed depending on the kit used.

Data source location Sabadell, Barcelona, Spain.

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Significant differences in let-7a expression were found between controls and CRC patients for kit N. The impact of PCR efficiency on Cqs. Bias, resolution, precision, and implications. Appendix A Supplementary data associated with this article can be found in the online version at doi: Published online Sep We found no significant differences between controls and patients for the other miRNAs analyzed.

Data on individual PCR efficiency values as quality control for circulating miRNAs

Evaluation of qPCR curve analysis methods for reliable biomarker discovery: Associated Data Supplementary Materials Supplementary material. Experimental factors CRC patients vs. Support Center Support Center. Supplementary material Supplementary material Click here lijregpcr view.

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Open in a separate window. Comparison of five commercial kits, doi: Exiqon redesigned the UniSp6 spike-in [2]. Experimental features miRNAs were extracted from plasma using five commercially available kits. National Center for Biotechnology InformationU.

The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

For miR, the mean Cq for the MN kit was Data linrefpcr Analyzed data. This is an open access article under the CC BY license http: This is especially important when the initial RNA quantity is so low that cannot be accurately quantified, such as in circulating RNA extractions.

The improvements resulted in a higher PCR efficiency 1. After isolating RNA with the five kits, we measured the abundance of four candidate miRNA biomarkers for colorectal cancer miR, miRa, let-7a, and miRa and miR as endogenous control.

For let-7a, PCR efficiency was suboptimal 1. Author information Article notes Copyright and License information Disclaimer. Journal List Data Brief v.

Data on individual PCR efficiency values as quality control for circulating miRNAs

Footnotes Appendix A Supplementary data associated with this article can be found in the online version at doi: Data was obtained by extracting plasma RNA from 8 patients 4 cancer and 4 healthy using five commercially available kits Figs. Caution must be taken to interpret this result because let-7a displayed low PCR efficiency 1.

Variability in microRNA recovery from plasma: Please review our privacy llinregpcr.